Technology Platform Light Microscopy
The Technology Platform Light Microscopy (TPLM) operates advanced light microscopes and image analysis resources and develops new technologies for light microscopy since 2022.
The TPLM provides access to light microscopes at the Centre for Structural Systems Biology (CSSB) in the Advanced Light and Fluorescence Microscopy (ALFM) Core Facility and at the Department of Biology with the Light Microscopy Platform at the Institute of Plant Science and Microbiology (IPM) and the Institute of Cell and Systems Biology of Animals (IZS).
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Available equipment
Photo: UHH/Feuerböther
SP8 confocal microscope with FALCON FLIM unit
Description: This inverted point-scanning confocal microscope from Leica is equipped with a 405 nm laser, a white light laser and 4 hybrid detectors. The microscope has an incubation unit for live cell imaging and a FALCON FLIM unit. The microscope is programmable via the Navigator module of the Leica LASX software. The microscope is operated in a biological safety level 2 (BSL2) laboratory.
Main applications: Confocal microscopy, FLIM, FRET, live cell microscopy.
Photo: UHH/Feuerböther
cryoCLEM widefield microscope
Description: This upright widefield microscope from Leica is equipped with a cryo-stage for imaging vitrified specimens on electron microscopy grids under constant cooling with liquid nitrogen. A mercury metal halide burner (EL6000) is available as the fluorescence excitation light source. The microscope is operated in a biological safety level 2 (BSL2) laboratory.
Main applications: Light cryo-microscopy, widefield microscopy, correlative light- and electron-cryo-microscopy.
Photo: UHH/Feuerböther
cryoCLEM Stellaris 8 confocal microscope
Description: This upright confocal microscope from Leica is equipped with a cryo-stage for imaging vitrified specimens on electron microscopy grids under constant cooling with liquid nitrogen. Available fluorescence excitation light sources include a 405 nm pulsed laser, a white light pulsed laser, and 488 nm, 561 nm, and 633 nm cw lasers. The microscope is equipped with four hybrid detectors and has a FLIM unit. In addition, the microscope can be operated in widefield mode and has LED a fluorescence widefield excitation light source. The microscope is programmable via the Navigator module of the Leica LASX software. The microscope is operated in a biological safety level 2 (BSL2) laboratory.
Main applications: Light cryo-microscopy, confocal microscopy, FLIM, FRET, widefield microscopy, correlative light- and electron-cryo-microscopy.
Photo: UHH/Feuerböther
DMi8 inverted widefield microscope for live cell imaging and with Primo Alveole micropatterning unit
Description: This inverted widefield microscope from Leica is equipped with an incubation unit for live cell measurements and an LED fluorescence excitation light source. In addition, a Primo Alveole micropatterning unit is attached. The microscope is operated in a biological safety level 2 (BSL2) laboratory.
Main applications: Live cell microscopy, widefield microscopy, phase contrast microscopy, DIC, micropatterning.
Photo: UHH/Feuerböther
DMi8 inverted widefield microscope for live cell imaging and THUNDER Software
Description: This inverted widefield microscope from Leica is equipped with an incubation unit for live cell measurements, an LED fluorescence excitation light source, and THUNDER software for computational clearing. The microscope is programmable for complex tasks, such as scanning multiwell plates. The microscope is operated in a biological safety level 2 (BSL2) laboratory.
Main applications: Live cell microscopy, widefield microscopy, phase contrast microscopy, DIC, scanning of multiwell plates.
Photo: UHH/Feuerböther
DM6 B upright widefield microscope #1
Description: This upright widefield microscope from Leica is equipped with a fluorescence excitation light source. The microscope is operated in a biological safety level 2 (BSL2) laboratory.
Main applications: Widefield microscopy, phase contrast microscopy, DIC, histology.
Photo: UHH/Feuerböther
DM6 B upright widefield microscope #2
Description: This upright widefield microscope from Leica is equipped with a fluorescence excitation light source and is fully motorized. The microscope is operated in a biological safety level 2 (BSL2) laboratory.
Main applications: Widefield microscopy, phase contrast microscopy, DIC, histology.
Photo: UHH/Feuerböther
DM6 B upright widefield microscope #3
Description: This upright widefield microscope from Leica is equipped with a fluorescence excitation light source. The microscope operated in the BSL2 lab of Tim Gilberger at CSSB Hamburg.
Main applications: Widefield microscopy, phase contrast microscopy, DIC, histology.
Photo: UHH/Feuerböther
SR GSD 3D Super-resolution microscope
Description: This inverted microscope from Leica is equipped for Single Molecule Localization Microscopy (SMLM) super-resolution microscopy and a special stage which is directly coupled to the objective. Fluorescence excitation can be performed in widefield mode or in Total Internal Reflection Fluorescence (TIRF) mode. The microscope is operated in a biological safety level 2 (BSL2) laboratory.
Main applications: Super-resolution microscopy, TIRF, STORM, DNA-PAINT.
Photo: UHH/Feuerböther
Spinning-Disc and SIM microscope with FRAP unit
Description: This inverted microscope from Nikon has multiple modes in which it can be operated. There is a Yokogawa CSU W1 spinning disc unit with an Andor iXon EMCCD camera and a Nikon E-SIM unit for Structured Illumination Microscopy (SIM). In addition, the microscope is equipped with a Fluorescence Recovery after Photobleaching (FRAP) unit from Rapp Optoelectronics. An incubation unit for live cell measurements is available. The microscope is fully programmable via the JOBS module in Nikon NIS Elements software. The microscope is operated in a biological safety level 2 (BSL2) laboratory.
Main applications: Spinning disc confocal microscopy, SIM, FRAP, photoactivation, live cell microscopy, scanning of multiwell plates.
Photo: UHH/Feuerböther
LSM880 Airyscan confocal microscope
Description: This inverted point-scanning confocal microscope from Zeiss has an Airyscan detector, which can be used to achieve an approximately 1.8-fold increase in resolution over normal confocal microscopes, and a spectral Imaging detector. It is equipped with an incubation unit for live cell measurements. The microscope is operated in a biological safety level 2 (BSL2) laboratory.
Main applications: Confocal microscopy, live cell microscopy, airyscan microscopy.
Photo: UHH/Feuerböther
Elyra PS.1 Super-resolution microscope
Description: This inverted super-resolution microscope from Zeiss can be used for Single Molecule Localization Microscopy (SMLM) microscopy and Structured Illumination Microscopy (SIM). Fluorescence excitation can be performed in widefield mode or in Total Internal Reflection Fluorescence (TIRF) mode. It is equipped with an incubation chamber for live cell measurements. The microscope is operated in a biological safety level 2 (BSL2) laboratory.
Main applications: Super-resolution microscopy, TIRF, STORM, PALM, DNA-PAINT, SIM, wide-field microscopy.
Photo: UHH/Feuerböther
Lattice light sheet microscope
Description: This lattice light sheet microscope from Zeiss is suitable for fast volumetric imaging of living cells with particularly low phototoxicity and simultaneously high local resolution. It features two Hamamatsu sCMOS cameras. The microscope is operated in a biological safety level 2 (BSL2) laboratory.
Main applications: Lattice light sheet microscopy, live cell microscopy.
Photo: UHH/Feuerböther
Cell culture laminar flow work bench and incubators
Description: The cell culture laminar flow work bench and two incubators are fully equipped to allow ALFM Facility users to prepare and pre-incubate their live cell samples before microscopy. The cell culture workbench and the incubators are located in a biological safety level 2 (BSL2) laboratory.
Main applications: Preparation of live cell samples for microscopy.
Photo: UHH/Feuerböther
M125C stereo microscope
Description: The M125C is a small stereo microscope from Leica with LED white light source. The microscope is operated in a biological safety level 2 (BSL2) laboratory.
Main applications: Inspection and preparation of microscopy specimens.
Photo: UHH/Feuerböther
Analysis computer
Description: The analysis computer has 768 GB of memory and two NVIDIA RTX A6000 graphics cards for processing and analyzing large amounts of image data. FIJI, Imaris, Arivis and Matlab image analysis software are available on the computer.
Main applications: Image processing and quantitative image analysis of microscopy data.